Endodontology

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 33  |  Issue : 3  |  Page : 144--148

Evaluation and comparison of the apical seal obtained with Biodentine after conditioning of root end with three different solutions using ultraviolet-visible spectrophotometer: An in vitro study


Sethuparvathi Anitha1, Liza George1, Josey Mathew1, Sinju Paul1, Tom Varghese1, RV Vineet2 
1 Department of Conservative Dentistry and Endodontics, Annoor Dental College, Muvattupuzha, Kerala, India
2 Kerala University of Health Sciences, Kerala, India

Correspondence Address:
Dr, Sethuparvathi Anitha
Alpha Dental Clinic, Pala, Kottayam, Kerala
India

Aim: The aim of the study is to evaluate and compare the apical seal obtained with Biodentine after conditioning of root end with three different solutions (HEBP, chitosan, and ethylenediaminetetraacetic acid [EDTA]). Materials and Method: Forty-eight maxillary incisors were selected and decoronated. Instrumentation was done with protaper rotary files up to F4 file and obturated with AH plus sealer (Dentsply, Germany) and Protaper gutta-percha cone using lateral condensation technique. The apical part of each root was resected at 90° to the long axis of the root for 3 mm, and retrograde cavity preparation was done in standardized dimensions. The teeth were randomly divided into four groups with 12 samples in each group and later subjected to a standard regimen of 3 ml of respective solutions for 5 min: Group I – 18% HEBP, Group II – 0.2% chitosan, Group III – 17% EDTA, Group IV – saline (control). All teeth were then restored with Biodentine (Septodont, USA). Samples were coated with two coats of nail varnish except in the apical 3 mm which will be immersed in 5 ml of 2% methylene blue dye and stored in incubator for 72 h. The nail varnish will be removed later, and the teeth will be immersed in 35% nitric acid for 72 h. The solutions were then filtered using a fine-grit filter paper and centrifuged at 2000 rpm for 1 min. The solutions thus collected will be used to determine absorbency in ultraviolet-visible spectrophotometer. Results: The mean values of absorbance were greatest for Group IV (saline) followed by Group III (17% EDTA), Group II (0.2% chitosan), and Group I (18% HEBP). Group IV (saline) and Group III (17% EDTA) have got statistically significant difference with all the other groups. Group II has got statistically significant difference with all the other groups except Group I. Conclusion: Within the limitations of the study, it was concluded that irrigation with newer agents significantly influenced the sealing ability of biodentine. Root-end irrigation with 18% HEBP and 0.2% chitosan showed the least microleakage when compared to 17% EDTA.


How to cite this article:
Anitha S, George L, Mathew J, Paul S, Varghese T, Vineet R V. Evaluation and comparison of the apical seal obtained with Biodentine after conditioning of root end with three different solutions using ultraviolet-visible spectrophotometer: An in vitro study.Endodontology 2021;33:144-148


How to cite this URL:
Anitha S, George L, Mathew J, Paul S, Varghese T, Vineet R V. Evaluation and comparison of the apical seal obtained with Biodentine after conditioning of root end with three different solutions using ultraviolet-visible spectrophotometer: An in vitro study. Endodontology [serial online] 2021 [cited 2022 Jul 7 ];33:144-148
Available from: https://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2021;volume=33;issue=3;spage=144;epage=148;aulast=Anitha;type=0