Cytotoxicity of two different intercanal medicaments on human gingival fibroblasts - A Laboratory study
Behnaz Barakatein1, Alireza Farhad2, Elham Shadmehr3, Hamidreza Mohammad Sharifi4, Masoud Mohammadi Hamidreza Mohamad Sharif5, Amin Davoudi6
1 Private Practice of Endodontic, Isfahan University of Medical Sciences, Isfahan, Iran
2 Dental Research Center, Department of Endodontics, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Endodontics, School of Dentistry, UCSF Preventive & Restorative Dental Science, San Francisco, California, USA
4 Post Graduate Student of Prosthodontics, Student Research Committee, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
5 Dentist, Private Practice, Isfahan, Iran
6 Department of Prosthodontics, School of Dentistry, Shahrekord University of Medical Sciences, Iran
Dr. Amin Davoudi
Department of Prosthodontics, School of Dentistry, Shahrekord University of Medical Sciences, Resalat Square, Shahrekord, 038-322322400
Source of Support: None, Conflict of Interest: None
Aim: Intracanal medicaments are often recommended during endodontic sessions to eliminate the necrotic debris and microorganisms. The aim of the present study was to observe the cytotoxicity of calcium hydroxide (CH) and colchicine (COL), at different concentrations, on human gingival fibroblast cells.
Materials and Methods: Human gingival fibroblasts were cultured on plastic flasks containing RPMI 1640 media and fetal calf serum 10% supplemented with antibiotic agents. Trypsin/ethylenediaminetetraacetic acid 0.2% enzyme was used to isolate the cells, and the suspension was transferred to tubes for centrifuging. Conventional CH and COL were separately mixed with sterile saline solution to prepare a stock media. By serial dilution of stock media, desired concentrations were prepared at 2, 1.75, 1.5, and 1.25 mg/ml, separately. After considering a control group, the cells were exposed to test materials. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted at 24 h, 72 h, and 7 days later. Optical density (OD) was evaluated to attain cell viability percentage. Finally, the recorded data were analyzed by Kruskal–Wallis and Mann–Whitney tests using SPSS software version 19 at a significant level of 0.05.
Results: The highest (1.40 ± 0.66) and lowest (0.15 ± 0.00) ODs were observed in CH 1.25 mg/ml and COL 1.5 mg/ml after 72 h, respectively. All of the concentrations of both CH and COL showed significant OD differences with the control group (all P = 0.001).
Conclusion: Both CH and COL manifested similar cytotoxicity on human gingival fibroblast cells.