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ARTICLE
Year : 1999  |  Volume : 11  |  Issue : 2  |  Page : 52-56

Antibacterial effect of various endodontic irrigants on select bacteria : An in-vitro study


1 Professor & Head, Dept. of Conservative Dentistry and Endodontics, Govt. Dental College & Hospital, Afzalgunj, Hyderabad, India
2 PG. Student, Dept. of Conservative Dentistry and Endodontics, Govt. Dental College & Hospital, Afzalgunj, Hyderabad, India

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Source of Support: None, Conflict of Interest: None


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This study was undertaken to test the antibacterial effect of six endodontic irrigants against five select bacteria. Infected pulp tissue was removed from the freshly extracted carious exposed teeth and used as specimen for isolation of bacteria. Streptococcus viridans, Streptococcus faecalis, Staphylococcus aureus, Pseudomonas auruginosa and Escherichia coli were selected from these bacterial isolations. Test irrigants were 0.2% chlorhexidine gluconate + 2.5% NaOCl, 0.2% chlorhexidine gluconate, 2.5% NaOCl, 3% H2O2, 10% EDTA, and 0.9% saline. Pre-sterilized 8 mm Whatman paper disks were soaked in the test irrigant and placed on the agar plates seeded with the test bacteria. After 24 hr. incubation at 37° C, inhibition zones were measured. Among the test irrigants, the combined use of 0.2% chlorhexidine gluconate and 2.5%NaOCl appeared to be the most effective antibacterial agent, 0.2% chlorhexidine and 2.5% NaOCl were moderately effective, 3% H2O2 and 10% EDTA were least effective and 0.9% saline proved totally ineffective.


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